product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ISL2 / Islet 2 Antibody (aa1-30) LS-C165303
catalog :
LS-C165303
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunohistochemistry - frozen section
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:400; fig 1i
Savier E, Eglen S, Bathélémy A, Perraut M, Pfrieger F, Lemke G, et al. A molecular mechanism for the topographic alignment of convergent neural maps. elife. 2017;6: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C165303 image 1
Fluorescent image of HUVEC cell stained with ISL2 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). ISL2 immunoreactivity is localized to Nucleus significantly.
image 2 :
LifeSpan Biosciences LS-C165303 image 2
ISL2 Antibody western blot of A2058 cell line lysates (35 ug/lane). The ISL2 antibody detected the ISL2 protein (arrow).
product information
AntibodyID :
172650
AntibodyName :
LS-C165303
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
ISL2 / Islet 2 Antibody (aa1-30) LS-C165303
Specificity :
This ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-30
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Gene :
ISL2 / Islet 2
BlockingPeptide :
LS-E4253
StandardGeneSymbol :
ISL2
Reactivity :
Human
Usage :
IF (1:10 - 1:50), WB (1:1000)
ShortWebDescription :
Islet 2 antibody LS-C165303 is an unconjugated rabbit polyclonal antibody to human Islet 2 (ISL2) (aa1-30). Validated for IF and WB. Cited in 1 publication.
Synonyms :
ISL2, ISL LIM homeobox 2, Islet-2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.