This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ABCB1 / MDR1 / P Glycoprotein Antibody (clone C219) LS-C16468
catalog :
LS-C16468
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C219

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
17209
AntibodyName :
LS-C16468
TargetSpecies :
Hamster
Host Species :
Mouse
Product Name :
ABCB1 / MDR1 / P Glycoprotein Antibody (clone C219) LS-C16468
Specificity :
Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein; not species specific. Species Crossreactivity: human, mouse, rat and hamster. Crossreactivity Proteins: a ~200kD protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2).
ClonalityDesc :
Monoclonal
CloneName :
C219
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.1% Sodium Azide, 1% BSA
ImmunogenType :
Cell fraction
ImmunogenDesc :
SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
ABCB1 / MDR1 / P Glycoprotein
StandardGeneSymbol :
ABCB1
gene family :
Transporter
Subfamily :
ATP-binding cassette - ABCB/MDR
Reactivity :
Mouse, Rat, Hamster, Human
Usage :
Flo, ICC, IHC, IHC-Fr, IHC-P, IP, WB (1:25 - 1:50)
ShortWebDescription :
P Glycoprotein antibody LS-C16468 is an unconjugated mouse monoclonal antibody to P Glycoprotein (ABCB1 / MDR1) from hamster. It is reactive with human, mouse, rat and other species. Validated for Flow, ICC, IHC, IP and WB.
UsageText :
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Cell permeabilization required. Western Blot: 1:25-1:50. Immunohistochemistry (Paraffin and Frozen): For paraffin sections, dilute 1:12.5 for biotin based detection. The antibody should be incubated for 60 minutes at RT. Immunocytochemistry: Cytospin preparations. Positive control: Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.
Synonyms :
ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.