product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
SMAD9 Antibody (aa200-228) LS-C161303
catalog :
LS-C161303
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 3f
Park D, Cheng J, McGrath J, Lim M, Cushman C, Swanson S, et al. Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. Nat Cell Biol. 2020;22:603-615 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C161303 image 1
SMAD9 Antibody western blot of A549 cell line lysates (35 ug/lane). The SMAD9 antibody detected the SMAD9 protein (arrow).
image 2 :
LifeSpan Biosciences LS-C161303 image 2
Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
product information
AntibodyID :
168623
AntibodyName :
LS-C161303
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
SMAD9 Antibody (aa200-228) LS-C161303
Specificity :
This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa200-228
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Gene :
SMAD9
BlockingPeptide :
LS-E3699
StandardGeneSymbol :
SMAD9
Reactivity :
Human
Usage :
IF (1:10 - 1:50), WB (1:1000)
ShortWebDescription :
SMAD9 antibody LS-C161303 is an unconjugated rabbit polyclonal antibody to human SMAD9 (aa200-228). Validated for IF and WB. Cited in 1 publication.
Synonyms :
SMAD9, MAD homolog 9, SMAD 9, SMAD8A, SMAD8, Smad9, MADH9, SMAD8B, MADH6, Mothers against DPP homolog 9, SMAD family member 9
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.