product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-SDC2 / Syndecan 2
catalog :
LS-C15755
quantity :
price :
clonality :
monoclonal
host :
mouse
reactivity :
cow
application :
western blot, ELISA, immunohistochemistry
more info or order :
LifeSpan Biosciences product webpage
product information
?AntibodyID :
16496
AntibodyName :
LS-C15755
TargetSpecies :
Bovine
Host Species :
Mouse
Product Name :
Anti-SDC2 / Syndecan 2
Specificity :
Recognizes bovine Heparan Sulfate Proteoglycan protein core of basement membrane at >200kD. Species cross-reactivity: Human, canine. No cross-reactivity with mouse, rat or amphibian tissues.
ClonalityDesc :
Monoclonal
PresentationDesc :
DMEM, 3% BSA. No preservatives added.
ImmunogenDesc :
Bovine Glomeruli Heparan Sulfate Proteoglycan.
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
IsotypeName :
IgG1
Gene :
SDC2 / Syndecan 2
StandardGeneSymbol :
SDC2
Reactivity :
Bovine, Dog, Human
Usage :
ELISA (1:50), WB (1:50 - 1:100)
UsageText :
Suitable for use in ELISA and Western Blot. Not suitable for IHC use on cultured cell. ELISA: 1:50. Western Blot: 1:50-1:100. Do not use milk as blocking agent. Must be digested with chondroitinase prior to running on SDS gels because undigested HSPG is too large for most gels. Treatment is at a concentration of chondroitinase of 10u/ml in Tris-HCl pH 8.0. Make tissue or cell extract in 20-50 mM Tris pH 7.6-8.0 with 0.15 M sodium chloride in the presence of protease inhibitors. Add 1 ul of enzyme to 30 ul of extract and incubate 30 minutes at 37?C. Add SDS sample buffer, heat or boil sample as normal for SDS.
Synonyms :
SDC2, CD362, CD362 antigen, HSPG, HSPG1, Fibroglycan, Syndecan proteoglycan 2, SYND2, Syndecan 2, Syndecan-2
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.