product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
APG5 / ATG5 Antibody (aa209-238) LS-C156610
catalog :
LS-C156610
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht M, et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron. 2021;109:299-313.e9 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C156610 image 1
Fluorescent image of U251 cells stained with ATG5 Antibody. Antibody was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 mu M, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
image 2 :
LifeSpan Biosciences LS-C156610 image 2
Cos7, HEK293, MEF, and HeLa cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
image 3 :
LifeSpan Biosciences LS-C156610 image 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
product information
AntibodyID :
163925
AntibodyName :
LS-C156610
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
APG5 / ATG5 Antibody (aa209-238) LS-C156610
Specificity :
This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa209-238
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Gene :
APG5 / ATG5
BlockingPeptide :
LS-E5603
StandardGeneSymbol :
ATG5
Reactivity :
Mouse, Human
Usage :
IF, IHC, IHC-P (1:50 - 1:100), WB (1:1000)
ShortWebDescription :
ATG5 antibody LS-C156610 is an unconjugated rabbit polyclonal antibody to ATG5 (APG5) (aa209-238) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Cited in 1 publication.
Synonyms :
ATG5, APG5, APG5-LIKE, APG5L, Apoptosis specific protein, Apoptosis-specific protein, Autophagy related 5, HAPG5, ASP, Autophagy protein 5
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.