This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
HSPB1 / HSP27 Antibody (Azide-free) LS-C15661
catalog :
LS-C15661
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
16402
AntibodyName :
LS-C15661
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
HSPB1 / HSP27 Antibody (Azide-free) LS-C15661
Specificity :
Recognizes human HSP27 at 24-27kD. Species cross-reactivity: Chimpanzee and monkey. Does not cross-react with mouse or rat.
ClonalityDesc :
Monoclonal
AntibodyModification :
Azide-free Unconjugated
PresentationDesc :
PBS, pH 7.4. No preservatives, no stabilizing proteins added.
ImmunogenType :
Cells
ImmunogenDesc :
Partially purified hsp27 protein from breast cancer MCF-7 cells. MW: 24-27kD. Cellular Localization: Cytoplasmic/nuclear.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG1
Gene :
HSPB1 / HSP27
StandardGeneSymbol :
HSPB1
Reactivity :
Human, Monkey
Usage :
IHC, IHC-P, WB (1 - 2 µg/ml)
ShortWebDescription :
HSP27 antibody LS-C15661 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1) from human. It is reactive with human and monkey. Validated for IHC and WB.
UsageText :
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1-2 ug/ml for 30 minutes at RT. Requires boiling tissue sections in10 mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes. Positive control: BT474, MDA-MB-453 cells, and breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.