This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Hemagglutinin / HA Tag Antibody (Biotin) LS-C154293
catalog :
LS-C154293
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
160968
AntibodyName :
LS-C154293
Host Species :
Rabbit
Product Name :
Hemagglutinin / HA Tag Antibody (Biotin) LS-C154293
Specificity :
This antibody has reacted with all HA tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
ClonalityDesc :
Polyclonal
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide.  A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in 100 µL of deionized water.
IsotypeName :
IgG
Gene :
Hemagglutinin / HA Tag
StandardGeneSymbol :
HA Tag
Usage :
ELISA (1:10000 - 1:50000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)
ShortWebDescription :
HA Tag antibody LS-C154293 is a biotin-conjugated rabbit polyclonal antibody to HA Tag (Hemagglutinin). Validated for ELISA, IHC and WB.
UsageText :
Anti-HA is optimally suited for monitoring the expression of HA tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- terminus of targeted proteins as is expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
, HA Tag
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.