antibody

RFP / Red Fluorescent Protein Antibody (Biotin) LS-C154289

LS-C154289

polyclonal

domestic rabbit

biotin

western blot, ELISA, immunohistochemistry

LS-C154289

Discosoma

Rabbit

RFP / Red Fluorescent Protein Antibody (Biotin) LS-C154289

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.

Polyclonal

Biotin

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Fusion protein

The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Affinity chromatography

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Reconstitute in 100 µL of deionized water.

IgG

RFP / Red Fluorescent Protein

Discosoma

ELISA (1:10000 - 1:80000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Red Fluorescent Protein antibody LS-C154289 is a biotin-conjugated rabbit polyclonal antibody to discosoma Red Fluorescent Protein (RFP). Validated for ELISA, IHC and WB.

Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Worldwide