This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ACTB / Beta Actin Antibody (aa350-375, HRP) LS-C154279
catalog :
LS-C154279
clonality :
polyclonal
host :
domestic rabbit
conjugate :
HRP
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
160954
AntibodyName :
LS-C154279
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
ACTB / Beta Actin Antibody (aa350-375, HRP) LS-C154279
Specificity :
A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
ClonalityDesc :
Polyclonal
AntibodyModification :
HRP
AntigenModification :
aa350-375
PresentationDesc :
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in 100 µL of deionized water.
IsotypeName :
IgG
Gene :
ACTB / Beta Actin
StandardGeneSymbol :
ACTB
Reactivity :
Mouse, Goat, Guinea pig, Sheep, Bovine, Rat, Hamster, Pig, Human, Monkey
Usage :
ELISA (1:20000), IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)
ShortWebDescription :
Beta Actin antibody LS-C154279 is an HRP-conjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa350-375) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
UsageText :
Beta Actin Antibody Peroxidase Conjugated antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kD in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.