antibody

DYKDDDDK Tag Antibody (HRP) LS-C154275

LS-C154275

polyclonal

domestic rabbit

HRP

western blot, ELISA, immunohistochemistry

LS-C154275

Rabbit

DYKDDDDK Tag Antibody (HRP) LS-C154275

This monoclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Polyclonal

HRP

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Purified protein

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.  Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling.  This antibody reacts with FLAG conjugated proteins.

Affinity purified

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Reconstitute in 100 µL of deionized water.

IgG

DYKDDDDK Tag

ELISA (1:20000), IHC (1:500 - 1:2000), WB (1:2000 - 1:5000)

DYKDDDDK Tag antibody LS-C154275 is an HRP-conjugated rabbit polyclonal antibody to DYKDDDDK Tag. Validated for ELISA, IHC and WB.

This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and DYKDDDDK containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. This polyclonal antibody to detect DYKDDDDK conjugated proteins binds DYKDDDDK containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

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