This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
V5 Tag Antibody LS-C154221
catalog :
LS-C154221
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
160896
AntibodyName :
LS-C154221
Host Species :
Rabbit
Product Name :
V5 Tag Antibody LS-C154221
Specificity :
This antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG
Gene :
V5 Tag
Usage :
ELISA (1:10000 - 1:60000), IHC (1:500 - 1:3000), WB (1:5000 - 1:10000)
ShortWebDescription :
V5 Tag antibody LS-C154221 is an unconjugated rabbit polyclonal antibody to V5 Tag. Validated for ELISA, IHC and WB.
UsageText :
Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Synonyms :
, V5 Epitope Tag, GKPIPNPLLGLDST
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.