product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
GFP Antibody (clone 9F9.F9) LS-C154208
catalog :
LS-C154208
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9F9.F9
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
more info or order :
LifeSpan Biosciences product webpage
citations: 4
Reference
Jesse S, Koenig A, Ellenrieder V, Menke A. Lef-1 isoforms regulate different target genes and reduce cellular adhesion. Int J Cancer. 2010;126:1109-20 pubmed publisher
Shen W, Da Silva J, He H, Cline H. Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo. J Neurosci. 2009;29:5032-43 pubmed publisher
Yang F, Camp D, Gritsenko M, Luo Q, Kelly R, Clauss T, et al. Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. J Cell Sci. 2007;120:4060-70 pubmed
Forrest J, Paden C, Allen R, Collins J, Speck S. ORF73-null murine gammaherpesvirus 68 reveals roles for mLANA and p53 in virus replication. J Virol. 2007;81:11957-71 pubmed
product information
AntibodyID :
160883
AntibodyName :
LS-C154208
TargetSpecies :
Aequorea victoria
Host Species :
Mouse
Product Name :
GFP Antibody (clone 9F9.F9) LS-C154208
Specificity :
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
ClonalityDesc :
Monoclonal
CloneName :
9F9.F9
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
PurificationDesc :
Protein A affinity chromatography
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG1,k
Gene :
GFP
StandardGeneSymbol :
GFP
Reactivity :
Aequorea victoria
Usage :
ELISA (1:10000 - 1:30000), Flo, IF, IHC (1:1000 - 1:5000), WB (1:3000 - 1:30000)
ShortWebDescription :
GFP antibody LS-C154208 is an unconjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, Flow, IF, IHC and WB. Cited in 4 publications.
UsageText :
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. Tested for cross reactivity with RFP only in ELISA.
Synonyms :
GFP
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.