This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
DYKDDDDK Tag Antibody (clone 29E4.G7, Agarose) LS-C154020
catalog :
LS-C154020
clonality :
monoclonal
host :
mouse
conjugate :
agarose
clone name :
29E4.G7
application :
western blot, immunoprecipitation
product information
AntibodyID :
160695
AntibodyName :
LS-C154020
Host Species :
Mouse
Product Name :
DYKDDDDK Tag Antibody (clone 29E4.G7, Agarose) LS-C154020
Specificity :
Anti-DYKDDDDK Affinity Gel binds the FLAG® epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide is recommended for competitive elution to recover fusion protein (see protocol).
ClonalityDesc :
Monoclonal
CloneName :
29E4.G7
AntibodyModification :
Agarose
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
RecommendedStorageDesc :
Store at 4°C.
IsotypeName :
IgG2a,k
Gene :
DYKDDDDK Tag
Usage :
IP, WB
ShortWebDescription :
DYKDDDDK Tag antibody LS-C154020 is an Agarose-conjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for IP and WB.
UsageText :
This agarose-conjugated antibody is optimally suited for immunoprecipitation and purification of DYKDDDDK tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. DYKDDDDK tag can be cleaved by the enterokinase because it contains the Enterokinase Cleavage Site (ECS). DO NOT USE A MAGNETIC STIR BAR WITH THIS PRODUCT. Do not use chaotropic reagents such as guanidine HCl; however urea may be used at less than 1 M concentration. Do not use any reagent that contains reducing agents such as DTT, DTE, or 2-mercaptoethanol with this product. Do not exceed 5% concentration of TWEEN 20 or TRITON X-100 with this product. Do not exceed 0.1% IGEPAL CA-630 or CHAPS. Do not exceed 0.2% digitonin. Do not exceed 1.0 M sodium chloride. DO NOT USE deoxycholate with this product. DO NOT USE SDS. Keep exposure to glycine HCl to less than 20 minutes.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.