This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
DYKDDDDK Tag Antibody (clone 29E4.G7, Biotin) LS-C154018
catalog :
LS-C154018
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
29E4.G7
application :
western blot, ELISA, immunohistochemistry, flow cytometry
product information
AntibodyID :
160693
AntibodyName :
LS-C154018
Host Species :
Mouse
Product Name :
DYKDDDDK Tag Antibody (clone 29E4.G7, Biotin) LS-C154018
Specificity :
This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
ClonalityDesc :
Monoclonal
CloneName :
29E4.G7
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in 100 µL of deionized water.
IsotypeName :
IgG2a,k
Gene :
DYKDDDDK Tag
Usage :
ELISA (1:100000), Flo, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)
ShortWebDescription :
DYKDDDDK Tag antibody LS-C154018 is a biotin-conjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ELISA, Flow, IHC and WB.
UsageText :
This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.