product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
MSLN / Mesothelin Antibody (clone MB-G10) LS-C154007
catalog :
LS-C154007
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MB-G10
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Onda M, Willingham M, Nagata S, Bera T, Beers R, Ho M, et al. New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clin Cancer Res. 2005;11:5840-6 pubmed
product information
AntibodyID :
160682
AntibodyName :
LS-C154007
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
MSLN / Mesothelin Antibody (clone MB-G10) LS-C154007
ClonalityDesc :
Monoclonal
CloneName :
MB-G10
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
PurificationDesc :
Protein A affinity chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
MSLN / Mesothelin
StandardGeneSymbol :
MSLN
Reactivity :
Human
Usage :
ELISA (1:10000 - 1:50000), IHC (1:100), WB (1:1000)
ShortWebDescription :
Mesothelin antibody LS-C154007 is an unconjugated mouse monoclonal antibody to human Mesothelin (MSLN). Validated for ELISA, IHC and WB. Cited in 1 publication.
UsageText :
This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
Synonyms :
MSLN, CAK1 antigen, MPF, SMRP, Mesothelin, CAK1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.