This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS-C154001
catalog :
LS-C154001
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
14E5.16C8.25F6

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry
product information
AntibodyID :
160676
AntibodyName :
LS-C154001
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS-C154001
Specificity :
This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.
ClonalityDesc :
Monoclonal
CloneName :
14E5.16C8.25F6
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.
PurificationDesc :
Protein A affinity chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
AKT1
StandardGeneSymbol :
AKT1
gene family :
Protein Kinase
Subfamily :
RAC/AKT
Reactivity :
Mouse, Rat, Chimpanzee, Human
Usage :
ELISA (1:20000), Flo, IHC (20 µg/ml), WB (1:500 - 1:3000)
ShortWebDescription :
AKT1 antibody LS-C154001 is an unconjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.
UsageText :
Anti-AKT Antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kD in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Synonyms :
AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.