This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
AKT1 Antibody (phospho-Ser473, clone 17F6.B11) LS-C153997
catalog :
LS-C153997
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
17F6.B11

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
product information
AntibodyID :
160672
AntibodyName :
LS-C153997
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
AKT1 Antibody (phospho-Ser473, clone 17F6.B11) LS-C153997
Specificity :
A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
ClonalityDesc :
Monoclonal
CloneName :
17F6.B11
AntibodyModification :
Unconjugated
AntigenModification :
pSer473
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
PurificationDesc :
Protein A affinity chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
AKT1
StandardGeneSymbol :
AKT1
gene family :
Protein Kinase
Subfamily :
RAC/AKT
Reactivity :
Mouse, Rat, Human, Monkey
Usage :
ELISA (1:20000), Flo, IF (1:500 - 1:3000), IHC (20 µg/ml), IP, WB (1:500 - 1:3000)
ShortWebDescription :
AKT1 antibody LS-C153997 is an unconjugated mouse monoclonal antibody to AKT1 (pSer473) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC, IP and WB.
UsageText :
Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Synonyms :
AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.