antibody

AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Atto 594) LS-C153792

LS-C153792

monoclonal

mouse

ATTO 594

phosphorylated

17F6.B11

The same clone is also sold as:

• Abnova: MAB1919
• Novus Biologicals: NBP1-69923
• Rockland Immunochemicals: 200-306-268, 200-355-268, 200-341-268, 200-343-268, 200-342-268, 200-301-B19, 200-301-268
• OriGene: TA319562, TA319558, TA319563

western blot, ELISA, immunocytochemistry, flow cytometry

LS-C153792

Human

Mouse

AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Atto 594) LS-C153792

This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

Monoclonal

17F6.B11

Atto 594

pSer473

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Synthetic peptide

Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.

Protein A affinity chromatography

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Reconstitute in 100 µL of deionized water.

IgG1,k

AKT1

AKT1

Protein Kinase

RAC/AKT

Mouse, Rat, Human, Monkey

ELISA (1:20000), FLISA (1:20000), Flo, IF (1:5000), WB (1:10000)

AKT1 antibody LS-C153792 is an Atto 594-conjugated mouse monoclonal antibody to AKT1 (pSer473) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, FLISA, Flow, IF and WB.

Anti-AKT pS473 Antibody ATTO 594 Conjugated is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Worldwide