antibody

AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Biotin) LS-C153789

LS-C153789

monoclonal

mouse

biotin

phosphorylated

17F6.B11

The same clone is also sold as:

• Novus Biologicals: NBP1-69923
• Abnova: MAB1919
• OriGene: TA319562, TA319563, TA319558
• Rockland Immunochemicals: 200-306-268, 200-301-B19, 200-343-268, 200-301-268, 200-342-268, 200-355-268, 200-341-268

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

LS-C153789

Human

Mouse

AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Biotin) LS-C153789

This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

Monoclonal

17F6.B11

Biotin

pSer473

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Synthetic peptide

Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.

Protein A affinity chromatography

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Reconstitute in 100 µL of deionized water.

IgG1,k

AKT1

AKT1

Protein Kinase

RAC/AKT

Mouse, Rat, Human, Monkey

ELISA (1:20000), Flo, IF (1:500 - 1:3000), IHC (20 µg/ml), IP, WB (1:500 - 1:3000)

AKT1 antibody LS-C153789 is a biotin-conjugated mouse monoclonal antibody to AKT1 (pSer473) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC, IP and WB.

Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Worldwide