This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
MAPK7 / ERK5 Antibody (phospho-Thr218/Tyr220) LS-C15060
catalog :
LS-C15060
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot
product information
AntibodyID :
15801
AntibodyName :
LS-C15060
TargetSpecies :
Human
Host Species :
Sheep
Product Name :
MAPK7 / ERK5 Antibody (phospho-Thr218/Tyr220) LS-C15060
Specificity :
Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pThr218/Tyr220
PresentationDesc :
100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
MAPK7 / ERK5
StandardGeneSymbol :
MAPK7
gene family :
Protein Kinase
Subfamily :
MAPK
Reactivity :
Human
Usage :
WB
UsageNotRecommended :
IHC-P
ShortWebDescription :
ERK5 antibody LS-C15060 is an unconjugated sheep polyclonal antibody to human ERK5 (MAPK7) (pThr218/Tyr220). Validated for WB.
UsageText :
Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).
Synonyms :
MAPK7, Big MAP kinase 1, BMK-1, Erk4, ERK5, MAPK 7, PRKM7, BMK1, BMK1 kinase, ERK-5, MAP kinase 7
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.