This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
MAPK3 / ERK1 Antibody (phospho-Thr185/Tyr187) LS-C15048
catalog :
LS-C15048
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot
product information
AntibodyID :
15789
AntibodyName :
LS-C15048
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
MAPK3 / ERK1 Antibody (phospho-Thr185/Tyr187) LS-C15048
Specificity :
Synthetic phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pThr185/Tyr187
PresentationDesc :
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from human ERK1/ERK2.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
MAPK3 / ERK1
StandardGeneSymbol :
MAPK3
gene family :
Protein Kinase
Subfamily :
MAPK
Reactivity :
Human
Usage :
WB
ShortWebDescription :
ERK1 antibody LS-C15048 is an unconjugated rabbit polyclonal antibody to human ERK1 (MAPK3) (pThr185/Tyr187). Validated for WB.
UsageText :
The antibody Peptide Competition. Extracts prepared from PC12 cells left unstimulated or stimulated with sorbitol. were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to. PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4?C, then. were incubated with ERK1&2 [pTpY185/187] antibody for two hours at room. temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide., the non-phosphopeptide corresponding to the immunogen, a generic. phosphothreonine-containing peptide, a generic phosphotyrosine-containing. peptide, or, the phosphopeptide immunogen. After washing, membranes were. incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Cat. # ALI4404) and. bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ERK1&2 [pTpY185/187] blocks. the antibody signal, thereby demonstrating the specificity of the antibody. The data. also show the induction of phosphorylation by the addition of sorbitol. y has been used for Western blot and immunostaining applications. Positive control: PC12 cells +/- Sorbitol.
Synonyms :
MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, MAP kinase 3, p44-ERK1, ERK1, PRKM3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.