antibody

Anti-GFAP Antibody (clone 542721) LS-C149956

LS-C149956

monoclonal

mouse

nonconjugated

542721

western blot, immunocytochemistry

LS-C149956

Human

Mouse

Anti-GFAP Antibody (clone 542721) LS-C149956

Human GFAP.

Monoclonal

542721

Unconjugated

Lyophilized from 0.2 um filtered solution in PBS, 5% trehalose

Recombinant protein

Purified, E. coli-derived recombinant human Glial Fibrillary Acidic Protein (rhGFAP; aa 292 - 432; Accession # P14136).

Protein G purified

Store at -20°C.

Sterile PBS. Resuspend to 500 ul

IgG1

GFAP

GFAP

Human

ICC (5 - 10 µg/ml), WB (1 - 2 µg/ml)

Immunocytochemistry-This antibody was used at a concentration of 5-10 µg/ml to detect human GFAP in astrocytes differentiated from neural progenitors. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1 % Triton X-100, and. 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red TM-coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells. were washed with PBS containing 0.1% BSA. Western blot-This antibody can be used at 1-2 µg/ml with the appropriate secondary reagents to detect human GFAP. Using a colorimetric detection system, the detection limit for rhGFAP is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGlo. Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human GFAP. The detection limit for rhGFAP is approximately 3 ng/well.

GFAP