antibody

BID Antibody LS-C148235

LS-C148235

polyclonal

domestic rabbit

nonconjugated

human, mouse

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

LS-C148235

Mouse

Rabbit

BID Antibody LS-C148235

Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by caspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kD C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.

Polyclonal

Unconjugated

Antiserum, 0.05% sodium azide

Recombinant protein

Full length recombinant mouse Bid protein.

Antiserum

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

BID

BID

Apoptosis

Mouse, Dog, Rat, Gerbil, Human

IHC, IHC-Fr, IHC-P (1:1000 - 1:5000), IP (1:50 - 1:200), WB (1:1000 - 1:2000)

BID antibody LS-C148235 is an unconjugated rabbit polyclonal antibody to BID from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.

BID, Apoptic death agonist, BID isoform L(2), BID isoform Si6, Bh3, FP497, p22 BID, BID isoform ES(1b), Desmocollin type 4, Human BID coding sequence

Worldwide