This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Alpha-Fetoprotein Antibody LS-C147809
catalog :
LS-C147809
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
152998
AntibodyName :
LS-C147809
TargetSpecies :
Rat
Host Species :
Sheep
Product Name :
Alpha-Fetoprotein Antibody LS-C147809
Specificity :
Rat AFP / Alpha-Fetoprotein. The reactivity of the antiserum is restricted to alpha-fetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-fetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a precipitation reaction has been observed with mouse amniotic fluid.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Purified protein
ImmunogenDesc :
Rat alpha fetoprotein
PurificationDesc :
Ion exchange chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Use 1 ml of sterile Distilled water.
Gene :
Alpha-Fetoprotein
StandardGeneSymbol :
AFP
Reactivity :
Rat
Usage :
ELISA, IF, IHC-P (1:1000), WB
ShortWebDescription :
Alpha-Fetoprotein antibody LS-C147809 is an unconjugated sheep polyclonal antibody to rat Alpha-Fetoprotein. Validated for ELISA, IF, IHC and WB.
UsageText :
This product is intended for use as unlabelled primary or secondary antibody reagent for the indirect detection of alpha fetoprotein in tissues and body fluids in immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000.
Synonyms :
AFP, Alpha-1-fetoprotein, Alpha-fetoprotein, HPAFP, Alpha-fetoglobulin, FETA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.