This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
GJA5 / CX40 / Connexin 40 Antibody (C-Terminus) LS-C14550
catalog :
LS-C14550
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
product information
AntibodyID :
15291
AntibodyName :
LS-C14550
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
GJA5 / CX40 / Connexin 40 Antibody (C-Terminus) LS-C14550
Specificity :
Reacts with the mouse Connexin 40 protein. On Western Blots, it identifies the target band at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence. Species cross-reactivity: Cross-reactivity with rat and human CX40 proteins is expected based on amino acid sequence homology.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide derived from the C-Terminus of the mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
GJA5 / CX40 / Connexin 40
StandardGeneSymbol :
GJA5
gene family :
Ion Channel
Subfamily :
Connexin
Reactivity :
Mouse
Usage :
IF (1 - 3 µg/ml), IHC, IHC-Fr, IP, WB (1 - 3 µg/ml)
ShortWebDescription :
Connexin 40 antibody LS-C14550 is an unconjugated rabbit polyclonal antibody to mouse Connexin 40 (GJA5 / CX40) (C-Terminus). Validated for IF, IHC, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunofluorescence: 1-3 ug/ml. Immunoprecipitation: 7 ug/reaction. Immunohistochemistry (Frozen sections): 1-3 ug/ml; For IHC, mice were perfused transcardially with 3 ml pre-fixative (50 mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin),. followed by 40 ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash (10% sucrose in 25 mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25 mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
Synonyms :
GJA5, Connexin 40, Connexin-40, Gap junction alpha-5 protein, ATFB11, CX40, Gap junction protein alpha 5
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
questions and comments