This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Nsr1p Antibody (clone 31C4) LS-C143060
catalog :
LS-C143060
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
31C4
application :
western blot, immunocytochemistry
product information
AntibodyID :
147839
AntibodyName :
LS-C143060
TargetSpecies :
Yeast
Host Species :
Mouse
Product Name :
Nsr1p Antibody (clone 31C4) LS-C143060
Specificity :
31C4 was isolated during a screen for monoclonal antibodies reactive with yeast nucleolar proteins. We determined the specificity by screening a yeast genomic library expressed as b-galactosidase fusion proteins in lgt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones b-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with EFEH, corresponding to amino acids 190 and onwards in Nsr1p. This peptide is ~20 amino acids into the first RRM domain. Thus, MCA-31C4 reacts with an epitope in the C-terminal 55% of Nsr1p. The antibody is provided as sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The IgG isotype and exact concentration are not known.
ClonalityDesc :
Monoclonal
CloneName :
31C4
AntibodyModification :
Unconjugated
PresentationDesc :
Tissue Culture Supernatant, 10 mM Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Nsr1p is localized in the nucleolus and binds to telomeric single-stranded oligonucleotide repeat sequence of the type and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
Nsr1p
Reactivity :
Yeast
Usage :
IF (1:500 - 1:5000), WB (1:10000)
ShortWebDescription :
Nsr1p antibody LS-C143060 is an unconjugated mouse monoclonal antibody to yeast Nsr1p. Validated for IF and WB.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.