This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD45 / LCA Antibody (clone BHPT-1, FITC) LS-C140357
catalog :
LS-C140357
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
BHPT-1

The same clone is also sold as:
reactivity :
human
application :
flow cytometry
product information
AntibodyID :
145124
AntibodyName :
LS-C140357
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CD45 / LCA Antibody (clone BHPT-1, FITC) LS-C140357
Specificity :
Human PTPRC / CD45
ClonalityDesc :
Monoclonal
CloneName :
BHPT-1
AntibodyModification :
FITC
PresentationDesc :
PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
ImmunogenDesc :
Peripheral blood monocytes from rheumatoid arthritis patient.
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Store at 4°C. Do not freeze.
IsotypeName :
IgG1
Gene :
CD45 / LCA
StandardGeneSymbol :
PTPRC
gene family :
Protein Phosphatase
Subfamily :
R1/R6
Reactivity :
Human
Usage :
Flo
ShortWebDescription :
LCA antibody LS-C140357 is an FITC-conjugated mouse monoclonal antibody to human LCA (CD45). Validated for Flow.
UsageText :
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 ul per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.