This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD45 / LCA Antibody (clone BHPT-1) LS-C140356
catalog :
LS-C140356
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BHPT-1

The same clone is also sold as:
reactivity :
human
application :
flow cytometry
product information
AntibodyID :
145123
AntibodyName :
LS-C140356
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CD45 / LCA Antibody (clone BHPT-1) LS-C140356
Specificity :
Human PTPRC / CD45
ClonalityDesc :
Monoclonal
CloneName :
BHPT-1
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.08% Sodium Azide
ImmunogenDesc :
Peripheral blood monocytes from rheumatoid arthritis patient.
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
IsotypeName :
IgG1
Gene :
CD45 / LCA
StandardGeneSymbol :
PTPRC
gene family :
Protein Phosphatase
Subfamily :
R1/R6
Reactivity :
Human
Usage :
Flo
ShortWebDescription :
LCA antibody LS-C140356 is an unconjugated mouse monoclonal antibody to human LCA (CD45). Validated for Flow.
UsageText :
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 ul per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light.
Synonyms :
PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.