product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
Ubiquitin Antibody (clone Ubi-1) LS-C140202
catalog :
LS-C140202
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Ubi-1
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:70,000; loading ...; fig 6
Noy S, Krawitz S, Del Bigio M. Chronic Traumatic Encephalopathy-Like Abnormalities in a Routine Neuropathology Service. J Neuropathol Exp Neurol. 2016;75:1145-1154 pubmed publisher
product information
AntibodyID :
144915
AntibodyName :
LS-C140202
TargetSpecies :
Bovine
Host Species :
Mouse
Product Name :
Ubiquitin Antibody (clone Ubi-1) LS-C140202
Specificity :
Ubiquitin
ClonalityDesc :
Monoclonal
CloneName :
Ubi-1
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide, 0.1% BSA
ImmunogenType :
Purified protein
ImmunogenDesc :
Purified bovine ubiquitin conjugated to carrier protein.
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 4°C, stable for 1 year.
IsotypeName :
IgG1
Gene :
Ubiquitin
Reactivity :
Bovine
Usage :
IF, IHC, IHC-Fr, IHC-P, IP, WB
ShortWebDescription :
Ubiquitin antibody LS-C140202 is an unconjugated mouse monoclonal antibody to bovine Ubiquitin. Validated for IF, IHC, IP and WB.
UsageText :
For immunohistology, immunofluorescence and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use lysis buffer in 10 mM N-ethylmaleimide (e. g. Pierce #23030) to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6 M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (pH 7.5)) for 30-60 minutes at 4°C, followed by extensive PBS washing.
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.