This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Macrophage Antibody (clone BM8) LS-C139904
catalog :
LS-C139904
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
BM8
reactivity :
mouse
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
144617
AntibodyName :
LS-C139904
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
Macrophage Antibody (clone BM8) LS-C139904
Specificity :
The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny.The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.The monoclonal antibody BM8 cross reacts with human heart macrophages.
ClonalityDesc :
Monoclonal
CloneName :
BM8
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide, 0.1% BSA
ImmunogenType :
Cells
ImmunogenDesc :
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 4°C.
IsotypeName :
IgG2a
Gene :
Macrophage
Reactivity :
Mouse, Human
Usage :
Flo, IHC, IHC-Fr, IHC-P, WB
ShortWebDescription :
Macrophage antibody LS-C139904 is an unconjugated rat monoclonal antibody to Macrophage from human and mouse. Validated for Flow, IHC and WB.
UsageText :
WB: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa; reduction with 2-mercaptoethanol destroys BM8 antigenF: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive controlFor flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The recommended use of this reagent is 10 ul per 250000 cells in a 100 ul total staining volume.
Synonyms :
, Mature Macrophage Marker
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.