This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
SELE / CD62E / E-selectin Antibody LS-C13897
catalog :
LS-C13897
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, immunohistochemistry - frozen section
product information
AntibodyID :
14638
AntibodyName :
LS-C13897
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
SELE / CD62E / E-selectin Antibody LS-C13897
Specificity :
Reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). Reacts with human endothelial cells activated with TNF-a, IL-1 or endotoxin. Found to react also with cells transfected with the E-selectin gene. Inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide, 0.1% BSA
ImmunogenType :
Recombinant protein
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG1
Gene :
SELE / CD62E / E-selectin
StandardGeneSymbol :
SELE
Reactivity :
Human, Monkey
Usage :
IHC, IHC-Fr, IP
ShortWebDescription :
E-selectin antibody LS-C13897 is an unconjugated mouse monoclonal antibody to E-selectin (SELE / CD62E) from human. It is reactive with human and monkey. Validated for IHC and IP.
UsageText :
The antibody is useful for staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey and baboon endothelial cells expressing E-selectin. Furthermore the antibody can be used for Immunoprecipitation and immunoassays. Immunohistology: 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-a (1 ng/ml), IL-1 (100U/ml) or LPS (1 ug/ml) in tissue culture medium at 37 C. As negative control it is advised to use a control murine IgG1 antibody.
Synonyms :
SELE, CD62E, ELAM, ESEL, E-selectin, LECAM2, CD62E antigen, ELAM-1, ELAM1, Selectin E
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.