This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ABCC2 / MRP2 Antibody (clone M2III-5, Concentrated) LS-C134870
catalog :
LS-C134870
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M2III-5

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
138551
AntibodyName :
LS-C134870
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
ABCC2 / MRP2 Antibody (clone M2III-5, Concentrated) LS-C134870
Specificity :
M2III-5 reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2III-5 was raised against a fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein. The Mab detects rat, mouse and human MRP2. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 orMRP5 gene products.
ClonalityDesc :
Monoclonal
CloneName :
M2III-5
AntibodyModification :
Concentrated Unconjugated
PresentationDesc :
Tissue Culture Supernatant, 0.1% Sodium Azide, 0.7% BSA, Serum-free
ImmunogenType :
Fusion protein
ImmunogenDesc :
Fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein.
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Store at 2-8°C.
IsotypeName :
IgG2b
Gene :
ABCC2 / MRP2
StandardGeneSymbol :
ABCC2
gene family :
Transporter
Subfamily :
ATP-binding cassette - ABCC/MRP
Reactivity :
Human
Usage :
Flo, ICC, IHC, IHC-Fr, IHC-P, WB
ShortWebDescription :
MRP2 antibody LS-C134870 is an unconjugated mouse monoclonal antibody to human MRP2 (ABCC2). Validated for Flow, ICC, IHC and WB.
UsageText :
M2III-5 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dilution. on acetone fixed cytospin preparations. For immunohistochemistry: M2III-5 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG (1:25, Dako) and a monoclonal mouse APAAP complex (1:50, Dako). M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 5 min 3 times. After incubation of M2III-5 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution, and anti-mouse-HRP.
Synonyms :
ABCC2, ABC30, CMOAT1, CMOAT, DJS, Multidrug resistance protein 2, MRP2, CMRP
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.