This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
GZMB / Granzyme B Antibody (clone GrB-7, Concentrated) LS-C134855
catalog :
LS-C134855
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GrB-7
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
138536
AntibodyName :
LS-C134855
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
GZMB / Granzyme B Antibody (clone GrB-7, Concentrated) LS-C134855
Specificity :
The monoclonal antibody reacts with the 33 kD human serine protease granzyme B. It does not react with human granzyme A. It is used as a marker for NK-cells and activated cytotoxic T-cells (CTL). This protease is localized in cytoplasmic granules and gives a granular staining pattern. Granzyme B is involved in target cell apoptosis during lymphocyte mediated cyto-toxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell will lead to induction of DNA fragmentation and apoptosis of the target cell.
ClonalityDesc :
Monoclonal
CloneName :
GrB-7
AntibodyModification :
Concentrated Unconjugated
PresentationDesc :
Tissue Culture Supernatant, 0.1% Sodium Azide, 1% BSA
ImmunogenType :
Native protein
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Store at 2-8°C.
IsotypeName :
IgG2a
Gene :
GZMB / Granzyme B
StandardGeneSymbol :
GZMB
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Human
Usage :
IHC, IHC-Fr, IHC-P, WB
ShortWebDescription :
Granzyme B antibody LS-C134855 is an unconjugated mouse monoclonal antibody to human Granzyme B (GZMB ). Validated for IHC and WB.
UsageText :
The monoclonal antibody stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. It does not react with other cell types. The antibody can not be used on frozen sections. The antibody is useful for staining granzyme B protein on Western blot. Before use in immunocytochemistry the slides have to be pretreated with 0.1 M sodium citrate, for 10 min. at 100°C (in microwave oven). A working dilution of 1:20 is advised but optimal dilution should be tested by serial dilution.
Synonyms :
GZMB, CCPI, CGL-1, Cathepsin G-like 1, CSP-B, CTLA-1, CTLA1, CGL1, CSPB, Fragmentin 2, GRB, HLP, Human lymphocyte protein, Fragmentin-2, SECT, Lymphocyte protease, T-cell serine protease 1-3E, C11, CTSGL1, Cytotoxic serine protease B, Granzyme B, Granzyme-2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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