This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PRSS3 / Trypsin 3 Antibody LS-C132707
catalog :
LS-C132707
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, neutralization
product information
AntibodyID :
136327
AntibodyName :
LS-C132707
TargetSpecies :
Human
Host Species :
Rat
Product Name :
PRSS3 / Trypsin 3 Antibody LS-C132707
Specificity :
Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96%, rat: 76%, mouse: 74%, dog: 72%. and chicken: 67%. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
NS0-derived recombinant human rhTrypsin-3.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
ResuspensionLiquidDesc :
Sterile buffer.
IsotypeName :
IgG2a
Gene :
PRSS3 / Trypsin 3
StandardGeneSymbol :
PRSS3
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Human
Usage :
Neut, WB (1 - 2 µg/ml)
ShortWebDescription :
Trypsin 3 antibody LS-C132707 is an unconjugated rat monoclonal antibody to human Trypsin 3 (PRSS3). Validated for Neut and WB.
UsageText :
Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15 ug/ml (IC 50) under conditions in which the enzyme was present at 0.03 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme. Western blot:1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.
Synonyms :
PRSS3, Brain trypsinogen, Mesotrypsin, PRSS4, Serine protease 4, Serine protease 3, TRY4, Trypsin III, Trypsin IV, Trypsinogen 4, MTG, Trypsinogen 5, Pancreatic trypsinogen III, Protease, serine, 3, Trypsin-3, Mesotrypsinogen, T9, TRY3, Trypsinogen IV
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.