This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Sialylated Mucin-Type-O-Glycans Antibody (clone FW17) LS-C132098
catalog :
LS-C132098
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FW17
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
135718
AntibodyName :
LS-C132098
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Sialylated Mucin-Type-O-Glycans Antibody (clone FW17) LS-C132098
Specificity :
Sialylated Mucin-Type-O-Glycans.
ClonalityDesc :
Monoclonal
CloneName :
FW17
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.4
ImmunogenDesc :
Purified mucus-glycoproteins from human amniotic fluid.
PurificationDesc :
Purified
RecommendedStorageDesc :
Lyophilized and resuspended forms: store at -20°C.
ResuspensionLiquidDesc :
Sterile ddH20 to 1 mg/ml
IsotypeName :
IgM
Gene :
Sialylated Mucin-Type-O-Glycans
Reactivity :
Human
Usage :
ELISA, ICC, IHC, IHC-P
ShortWebDescription :
Sialylated Mucin-Type-O-Glycans antibody LS-C132098 is an unconjugated mouse monoclonal antibody to human Sialylated Mucin-Type-O-Glycans. Validated for ELISA, ICC and IHC.
UsageText :
Tumor and normal tissues were first fixed in 6% neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 µm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2% H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5% in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2% in PBS), (4) swine anti-rabbit immunoglobulin (2% in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/ml - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01% H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21°C for 30 min.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.