This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
ALCAM / CD166 Antibody (aa28-527, Biotin) LS-C125325
catalog :
LS-C125325
clonality :
monoclonal
host :
mouse
conjugate :
biotin
reactivity :
human
application :
western blot, flow cytometry
product information
AntibodyID :
128945
AntibodyName :
LS-C125325
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
ALCAM / CD166 Antibody (aa28-527, Biotin) LS-C125325
Specificity :
Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, rmMadCAM or rmOCAM.
ClonalityDesc :
Monoclonal
AntibodyModification :
Biotin
AntigenModification :
aa28-527
PresentationDesc :
Sterile-filtered, lyophilized powder in PBS, pH 7.2, BSA
ImmunogenDesc :
Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM; aa 28-527; Accession No. Q13740).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS.
IsotypeName :
IgG1
Gene :
ALCAM / CD166
StandardGeneSymbol :
ALCAM
Reactivity :
Human
Usage :
Flo (25 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
CD166 antibody LS-C125325 is a biotin-conjugated mouse monoclonal antibody to human CD166 (ALCAM) (aa28-527). Validated for Flow and WB.
UsageText :
Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
ALCAM, CD166 antigen, MEMD, CD166
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.