This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
AKT1 Antibody (aa2-480) LS-C125031
catalog :
LS-C125031
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, flow cytometry
product information
AntibodyID :
128651
AntibodyName :
LS-C125031
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
AKT1 Antibody (aa2-480) LS-C125031
Specificity :
Recognizes human Akt1. Does not detect rhAkt2 or rhAkt3.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa2-480
PresentationDesc :
Lyophilized from Sterile Solution PBS, 5% Trehalose
ImmunogenDesc :
Sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS.
IsotypeName :
IgG1
Gene :
AKT1
StandardGeneSymbol :
AKT1
gene family :
Protein Kinase
Subfamily :
RAC/AKT
Reactivity :
Human
Usage :
ELISA, Flo (50 µg/ml), ICC (8 - 25 µg/ml), WB
ShortWebDescription :
AKT1 antibody LS-C125031 is an unconjugated mouse monoclonal antibody to human AKT1 (aa2-480). Validated for ELISA, Flow, ICC and WB.
UsageText :
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Suitable for use in Flow cytometry: 50 ug/ml. This antibody was validated for flow cytometry using MCF-7 cells. For. intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 20ulL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunocytochemistry: 8-25 ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells.
Synonyms :
AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.