This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CASP3 / Caspase 3 Antibody LS-C12481
catalog :
LS-C12481
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot
product information
AntibodyID :
13222
AntibodyName :
LS-C12481
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
CASP3 / Caspase 3 Antibody LS-C12481
Specificity :
Recognizes full-length Caspase 3 (Yama/Apopain) at 32kD and proteolytic fragments as demonstrated after in vitro cleavage of recombinant Caspase 3. Cross-reacts with human, mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.07 M Tris-Glycine, pH 7.4, 0.105 M NaCl, 0.035% Sodium Azide, 30% Glycerol
ImmunogenType :
Fusion protein
ImmunogenDesc :
Human full-length Caspase 3 fusion protein containing a histidine-6 tag.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
CASP3 / Caspase 3
StandardGeneSymbol :
CASP3
gene family :
Protease
Subfamily :
Cysteine C14
Reactivity :
Mouse, Rat, Human
Usage :
WB (0.5 - 2 µg/ml)
ShortWebDescription :
Caspase 3 antibody LS-C12481 is an unconjugated rabbit polyclonal antibody to Caspase 3 (CASP3) from human. It is reactive with human, mouse and rat. Validated for WB.
UsageText :
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml (will detect the 32kD precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells; previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20 ug per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blot Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Synonyms :
CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.