This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CASP3 / Caspase 3 Antibody (N-Terminus) LS-C12477
catalog :
LS-C12477
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
13218
AntibodyName :
LS-C12477
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
CASP3 / Caspase 3 Antibody (N-Terminus) LS-C12477
Specificity :
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide (KLH coupled) corresponding to N-Terminus residues adjacent to (Asp175) in human caspase-3.
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
CASP3 / Caspase 3
StandardGeneSymbol :
CASP3
gene family :
Protease
Subfamily :
Cysteine C14
Reactivity :
Human
Usage :
Flo (1:200), IF, IHC, IHC-Fr, IHC-P, IP (1:50), WB (1:1000)
ShortWebDescription :
Caspase 3 antibody LS-C12477 is an unconjugated rabbit monoclonal antibody to human Caspase 3 (CASP3) (N-Terminus). Validated for Flow, IF, IHC, IP and WB.
UsageText :
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Frozen): 1:200 using 3% formaldehyde as fixative. Immunohistochemistry (Paraffin): 1:200. Antigen retrieval: heat in citrate buffer, pH 8.0. Immunofluorescence (IC): 1:200. Flow Cytometry: 1:200.
Synonyms :
CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.