This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
TP53 / p53 Antibody (aa181-190, clone DO-11) LS-C11558
catalog :
LS-C11558
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DO-11

The same clone is also sold as:
reactivity :
human
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
12299
AntibodyName :
LS-C11558
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
TP53 / p53 Antibody (aa181-190, clone DO-11) LS-C11558
Specificity :
Recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kD cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells. DO-11 binds to an epitope within the central region of p53 between amino acids defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promoter usage, the epitope recognized by clone DO-11 is present in all isoforms. Recognizes mutant forms of p53 and has been used successfully for detection of p53 by western blotting.
ClonalityDesc :
Monoclonal
CloneName :
DO-11
AntibodyModification :
Unconjugated
AntigenModification :
aa181-190
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human p53 (AA 181-190).
PurificationDesc :
Purified IgG
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
TP53 / p53
StandardGeneSymbol :
TP53
gene family :
P53
Reactivity :
Human
Usage :
IHC-Fr, IHC-P (1:1000), IP, WB (1:1000)
ShortWebDescription :
P53 antibody LS-C11558 is an unconjugated mouse monoclonal antibody to human p53 (TP53) (aa181-190). Validated for IHC, IP and WB.
UsageText :
Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Synonyms :
TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.