This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
TP53 / p53 Antibody (aa20-25, clone DO-7) LS-C11556
catalog :
LS-C11556
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DO-7

The same clone is also sold as:
reactivity :
human
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
12297
AntibodyName :
LS-C11556
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
TP53 / p53 Antibody (aa20-25, clone DO-7) LS-C11556
Specificity :
Recognizes the human 53kD p53 tumor suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramerization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and, DNA repair and potentially apoptosis through interaction with,, and. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent systemrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple lacking the N-terminal region.
ClonalityDesc :
Monoclonal
CloneName :
DO-7
AntibodyModification :
Unconjugated
AntigenModification :
aa20-25
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human p53 (AA 20-25)
PurificationDesc :
Purified IgG
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG2b
Gene :
TP53 / p53
StandardGeneSymbol :
TP53
gene family :
P53
Reactivity :
Human
NonReactiveSpecies :
Mouse, Rat
Usage :
Flo (1:50 - 1:100), IHC-Fr, IHC-P (1:1000), IP, WB (1:1000)
ShortWebDescription :
P53 antibody LS-C11556 is an unconjugated mouse monoclonal antibody to human p53 (TP53) (aa20-25). Validated for Flow, IHC, IP and WB.
UsageText :
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 whole blood in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Colon or breast carcinoma.
Synonyms :
TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.