This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
UCHL1 / PGP9.5 Antibody (clone 31A3) LS-C11453
catalog :
LS-C11453
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
31A3

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
12194
AntibodyName :
LS-C11453
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
UCHL1 / PGP9.5 Antibody (clone 31A3) LS-C11453
Specificity :
Recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubiquitin pathway. Stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES. Clones 31A3 and each recognize a different epitope towards the N-terminus of the protein. Also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs. Has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue.
ClonalityDesc :
Monoclonal
CloneName :
31A3
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Native protein
ImmunogenDesc :
Native, from brain
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
UCHL1 / PGP9.5
StandardGeneSymbol :
UCHL1
gene family :
Protease
Subfamily :
Cysteine C12
Reactivity :
Human
Usage :
ELISA (1:500 - 1:2000), IHC, IHC-P (1:100 - 1:400), WB
ShortWebDescription :
PGP9.5 antibody LS-C11453 is an unconjugated mouse monoclonal antibody to human PGP9.5 (UCHL1). Validated for ELISA, IHC and WB.
UsageText :
For IHC-P specimens which have not been fixed in acetic acid/alcohol prior to embedding will require pretreatment using the microwave-citrate buffer method.
Synonyms :
UCHL1, Ubiquitin C-terminal hydrolase, PARK5, PGP9.5, PGP95, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Uch-L1, Ubiquitin carboxyl-terminal esterase L1, HEL-117, NDGOA, HEL-S-53, SPG79
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.