product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PLK1 / PLK-1 Antibody (clone OTI3F12) LS-C114491
catalog :
LS-C114491
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
OTI3F12
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Donizy P, Pagacz K, Marczuk J, Fendler W, Maciejczyk A, Halon A, et al. Upregulation of FOXP1 is a new independent unfavorable prognosticator and a specific predictor of lymphatic dissemination in cutaneous melanoma patients. Onco Targets Ther. 2018;11:1413-1422 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-C114491 image 1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
image 2 :
LifeSpan Biosciences LS-C114491 image 2
IHC of paraffin-embedded liver tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).
image 3 :
LifeSpan Biosciences LS-C114491 image 3
Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
product information
AntibodyID :
117258
AntibodyName :
LS-C114491
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PLK1 / PLK-1 Antibody (clone OTI3F12) LS-C114491
Specificity :
Human PLK / PLK1
ClonalityDesc :
Monoclonal
CloneName :
OTI3F12
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
ImmunogenType :
Transfected Cells
ImmunogenDesc :
Protein expressed in 293T cell transfected with human PLK1 expression vector.
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
PLK1 / PLK-1
StandardGeneSymbol :
PLK1
gene family :
Protein Kinase
Subfamily :
Polo
Reactivity :
Human
Usage :
Flo (1:100), IHC, IHC-P (1:150), IP, WB (1:2000)
ShortWebDescription :
PLK-1 antibody LS-C114491 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1). Validated for Flow, IHC, IP and WB. Cited in 1 publication.
Synonyms :
PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.