product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
CCL2 / MCP1 Antibody LS-C104751
catalog :
LS-C104751
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, ELISA
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Raikwar N, Braverman C, Snyder P, Fenton R, Meyerholz D, Abboud F, et al. Renal denervation and CD161a immune ablation prevent cholinergic hypertension and renal sodium retention. Am J Physiol Heart Circ Physiol. 2019;317:H517-H530 pubmed publisher
product information
AntibodyID :
106601
AntibodyName :
LS-C104751
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
CCL2 / MCP1 Antibody LS-C104751
Specificity :
Rat MCP-1
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Rat MCP-1.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex.
Gene :
CCL2 / MCP1
StandardGeneSymbol :
CCL2
gene family :
Intercrine
Subfamily :
Intercrine beta
Reactivity :
Rat
Usage :
ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
MCP1 antibody LS-C104751 is an unconjugated rabbit polyclonal antibody to rat MCP1 (CCL2). Validated for ELISA and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. Frozen samples fixed in 4% PFA. The primary antibody was incubated at 1 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat MCP-1 (MCAF) (100 ng/ml), a concentration of 4-6 ug/ml of this antibody is required. ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (LS-C104762) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.