This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
TNF Alpha Antibody (Biotin) LS-C104719
catalog :
LS-C104719
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106569
AntibodyName :
LS-C104719
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
TNF Alpha Antibody (Biotin) LS-C104719
Specificity :
Human TNF-alpha
ClonalityDesc :
Polyclonal
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human TNF-alpha.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Sterile PBS containing 0.1% BSA
Gene :
TNF Alpha
StandardGeneSymbol :
TNF
gene family :
TNF
Reactivity :
Human
Usage :
ELISA (0.25 - 1 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
TNF Alpha antibody LS-C104719 is a biotin-conjugated rabbit polyclonal antibody to human TNF Alpha. Validated for ELISA and WB.
UsageText :
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human TNF-alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-alpha (LS-C104377) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.