product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IL-33 Antibody LS-C104697
catalog :
LS-C104697
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Wang W, Li Y, Lv Z, Chen Y, Li Y, Huang K, et al. Bronchial Allergen Challenge of Patients with Atopic Asthma Triggers an Alarmin (IL-33, TSLP, and IL-25) Response in the Airways Epithelium and Submucosa. J Immunol. 2018;201:2221-2231 pubmed publisher
product information
AntibodyID :
106547
AntibodyName :
LS-C104697
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IL-33 Antibody LS-C104697
Specificity :
Human IL-33
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human IL-33.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile water
Gene :
IL-33
StandardGeneSymbol :
IL33
gene family :
Interleukin
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
IL-33 antibody LS-C104697 is an unconjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA and WB. Cited in 2 publications.
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 ug/ml-0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-33 (1 ng/ml), a concentration of 0.2-0.4 ug/ml of this antibody is required. ELISA: To detect hIL-33 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 (LS-C104696) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.