product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
CCL5 / RANTES Antibody LS-C104689
catalog :
LS-C104689
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA
more info or order :
citations: 1
product information
AntibodyID :
106539
AntibodyName :
LS-C104689
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
CCL5 / RANTES Antibody LS-C104689
Specificity :
Mouse RANTES
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Murine RANTES.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
100 ul sterile ddH20
Gene :
CCL5 / RANTES
StandardGeneSymbol :
CCL5
gene family :
Intercrine
Reactivity :
Mouse
Usage :
ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
RANTES antibody LS-C104689 is an unconjugated rabbit polyclonal antibody to mouse RANTES (CCL5). Validated for ELISA and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: This antibody stained colchicine injected mouse brain (somatosensory cortex) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/ml), a concentration of 0.8-1.2 ug/ml of this antibody is required. ELISA: To detect mRANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RANTES (LS-C104424) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mRANTES. Western Blot: To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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