antibody

CSF1 / MCSF Antibody LS-C104656

LS-C104656

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA

LS-C104656

Human

Rabbit

CSF1 / MCSF Antibody LS-C104656

Human M-CSF

Polyclonal

Unconjugated

Lyophilized from PBS, pH 7.2

Cell extract

E.coli derived recombinant Human M-CSF.

Immunoaffinity purified

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Reconstitute in sterile water to 0.1-1 mg/ml.

CSF1 / MCSF

CSF1

Hormone

Human

ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)

MCSF antibody LS-C104656 is an unconjugated rabbit polyclonal antibody to human MCSF (CSF1). Validated for ELISA and WB. Cited in 1 publication.

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hM-CSF (7 ng/ml), a concentration of 0.02-0.03 ug/ml of this antibody is required. ELISA: To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (LS-C104655) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

CSF1, CSF-1, MCSF, M-CSF, Lanimostim, CSFM

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