antibody

IL8 / Interleukin 8 Antibody LS-C104558

LS-C104558

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA

LS-C104558

Human

Rabbit

IL8 / Interleukin 8 Antibody LS-C104558

Human IL-8

Polyclonal

Unconjugated

Lyophilized from PBS, pH 7.2

Cell extract

E.coli derived recombinant Human IL-8.

Immunoaffinity purified

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Reconstitute with sterile water to 0.1-1.0 mg/ml.

IL8 / Interleukin 8

CXCL8

Intercrine

Intercrine alpha

Human

ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)

Interleukin 8 antibody LS-C104558 is an unconjugated rabbit polyclonal antibody to human Interleukin 8 (IL8). Validated for ELISA and WB. Cited in 1 publication.

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-8 (100ng/ml), a concentration of 2-3 ug/ml of this antibody is required. ELISA: To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-8 (LS-C104790) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-8. Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

CXCL8, 3-10C, AMCF-I, B-ENAP, C-X-C motif chemokine 8, GCP-1, IL-8, IL8, Interleukin-8, K60, LECT, GCP1, LUCT, NAF, NAP1, Neutrophil-activating factor, Interleukin 8, Protein 3-10C, MDNCF, TSG-1, Emoctakin, LYNAP, MONAP, NAP-1, SCYB8, T-cell chemotactic factor

Worldwide