This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IL-10 Antibody (clone 15B9-1-2) LS-C104515
catalog :
LS-C104515
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
15B9-1-2
reactivity :
human, mouse
application :
western blot, ELISA
product information
AntibodyID :
106365
AntibodyName :
LS-C104515
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
IL-10 Antibody (clone 15B9-1-2) LS-C104515
Specificity :
Mouse IL-10
ClonalityDesc :
Monoclonal
CloneName :
15B9-1-2
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Murine IL-10.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile water to a concentration of 1 mg/ml.
IsotypeName :
IgG2a
Gene :
IL-10
StandardGeneSymbol :
IL10
gene family :
Hormone
Reactivity :
Mouse
Usage :
ELISA (4 - 8 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
IL-10 antibody LS-C104515 is an unconjugated rat monoclonal antibody to mouse IL-10. Validated for ELISA and WB.
UsageText :
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/ml), a concentration of 3-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (LS-C104822) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1-2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2-4 ng/lane, under reducing or non-reducing conditions.
Synonyms :
IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.